目的: 研究制川乌与川贝母、浙贝母配伍前后其化学成分的整体变化情况, 从而分析乌头反贝母的相反机制。方法: 使用UPLC-ESI-LTQ-MS对配伍前后的煎煮液进行分析, 采用主成分分析(PCA)对数据进行分析并采用多级串联质谱技术对化学标志物进行鉴定。结果: 制川乌与川贝母、浙贝母配伍前后成分均有明显差异, 其中与川贝母配伍产生5个化学标志物, 与浙贝母配伍产生6个化学标志物, 重复出现的标志物为次乌头碱和苯甲酰基次乌头原碱。结论: 制川乌与两种贝母配伍后的化学标志物差异较大, 仅有2个相同, 结合前期的急性毒性试验结果, 推测制川乌与不同贝母配伍后LD₅₀的变化与其配伍后化学成分的变化相关; 从而说明相反不完全是通过其化学成分变化引起的, 其相反机制可能还通过其他因素共同引起。

Objective: To investigate the compatibility taboo theory, the systemic changes of Aconiti Radix Cocta in compatibility process with Fritillariae Cirrhosae Bulbus and Fritillariae Thunbergii Bulbus have been studied. Methods: Using UPLC-ESI-LTQ-MS to analyze the decoctions before and after compatibility, PCA was applied to comparing LC-MS data, then MSn was employed to identify the chemical markers. Results: There were significant differences before and after Aconiti Radix Cocta combined with Fritillariae Cirrhosae Bulbus and Fritillariae Thunbergii Bulbus, there were five chemcial markers in Aconiti Radix Cocta and Fritillariae Cirrhosae Bulbus taboo, and six in Aconiti Radix Cocta and Fritillariae Thunbergii Bulbus taboo, only hypaconitine and benzoylhypaconine were repeated as the same of two pairs. Conclusion: According to the research results of the chemical substance base and the acute toxicity, the changes of LD₅₀ value befer and after drug-pair compatibility may be from the difference of chemical markers between Aconiti Radix Cocta- Fritillariae Cirrhosae Bulbus and Aconiti Radix Cocta-Fritillariae Thunbergii Bulbus. The compatibility taboo theory may cause by other factors but not the chemical constituents changes.

国家重点基础研究发展计划(“973”计划)资助项目(2011CB505303);国家自然科学基金资助项目(81073040)