[关键词]
[摘要]
目的: 考察生地黄不同炮制阶段所含地黄寡糖和梓醇量的变化。方法: 采用水为溶媒, 提取不同炮制阶段生地黄中寡糖类成分, 流动相用乙腈-水(72∶28), 体积流量1.0 mL/min, NH2色谱柱分离, 示差折光检测器检测, HPLC法测定; 采用甲醇为溶媒, 超声提取梓醇, 流动相用甲醇-0.1%磷酸溶液(1∶99), 体积流量1.0 mL/min, C18色谱柱分离, 检测波长210 nm, HPLC法测定。结果: 不同炮制阶段生地黄中的寡糖成分及其量不同, 梓醇的量不同。结论: 炮制改变了生地黄中寡糖成分类型及其量, 同时也影响梓醇的量。
[Key word]
[Abstract]
Objective: To investigate the contents of oligosaccharide and catalpol in Rehmanniae Radix at various processing phases.Methods: Saccharide ingredient was extracted by ultrosonic extraction when water was used as solvent, separated by NH2chromatographic column while acetonitrile-water (72∶28) as mobile phase at flow rate of 1.0 mL/min, detected by differentialrefractive detector, and assayed by HPLC. Catalpinol was extracted by ultrosonic extraction when methanol was used as solvent,separated by C18 chromatographic column while methanol-0.1% phosphoric acid (1∶99)as mobile phase at flow rate of 1.0 mL/min, detected at wavelength of 210 nm, and assayed by HPLC. Results: There are significantdifferences at various processing phases of oligosaccharide and catalpol contents in Rehmanniae Radix. Conclusion: Processing could change the content and composition pattern of oligosaccharide in Rehmanniae Radix and affect the content of catalpol in Rehmanniae Radix at the same time.
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[基金项目]
国家自然科学基金资助项目(30672643,30472186,30772773);甘肃省自然科学基金资助项目(3ZS051-A25-078)