目的: 研究蛇足石杉Huperzia serrata的化学成分。方法: 利用硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法、中压柱色谱及半制备高效液相色谱等方法分离纯化;通过核磁共振谱、质谱等光谱数据鉴定化合物结构。结果: 分离得到10个化合物, 分别鉴定为β-谷甾醇(1)、5,7,4′-三羟基-3′-甲氧基黄酮(2)、5,7,4′-三羟基-3′,5′-二甲氧基黄酮(3)、芹菜素(4)、正三十烷醇(5)、21β-hydroxy-serrat-14-en-3β-ol(6)、21α-hydroxy-serrat-14-en-3β-ol(7)、16-oxo-21β-hydroxy-serrat-14-en-3α-yl-acetate(8)、3β,21α-dihydroxy-serrat-14-en-24-ol(9)、3α,21β-dihydroxy-serrat-14-en-24-ol(10)。结论: 化合物2～5为首次从本植物中分离得到。

Objective: To investigate the chemical constituents of Huperzia serrata. Methods: The constituents were isolated by silica gel column, Sephadex LH-20 gel column, medium pressure column, and semi-preparative HPLC chromatographies, and their structures were elucidated by chemical properties and spectroscopic analyses. Results The structures of ten compounds were isolated and identified as β-sitosterol (1), 5, 7, 4′-trihydroxy-3′-methoxyflavone (2), 5, 7, 4′-Trihydroxy-3′, 5′-dimethoxyflavone (3), apigenin(4), n-triacontanol (5), 21β-hydroxy-serrat-14-en-3β-ol (6), 21α-hydroxy-serrat-14-en-3β-ol (7), 16-oxo-21β-hydroxy-serrat-14-en-3α-yl-acetate (8), 3β, 21α-dihydroxy-serrat-14-en-24-ol (9), and 3α, 21β-dihydroxy-serrat-14-en-24-ol (10). Conclusion: Compounds 2—5 are obtained from this plant for the first time.