[关键词]
[摘要]
目的 优选白花刺参的最佳回流提取工艺条件。方法 采用UV法和HPLC法测定总皂苷及刺参苷J的量,以总皂苷提取率、刺参苷J提取率和干浸膏收率为综合评价指标,采用U8(84) 均匀设计表设计试验,考察乙醇体积分数、乙醇用量、提取次数、提取时间4种因素对总皂苷提取的影响。结果 白花刺参的最佳提取工艺参数:80%乙醇回流提取2次,每次1 h。结论 均匀设计法优选出的白花刺参总皂苷的提取工艺合理可行,为白花刺参总皂苷的进一步研究提供参考。
[Key word]
[Abstract]
Objective To optimize the extractive condition of Morina nepalensis by hot refluxing. Methods HPLC and UV were established to determine the monepaloside J and total saponins. U8(84) Uniform design form was selected as the experiments’ schedule, the total saponins, monepaloside J and the yield was used as evaluation indicator to and the ethanol concentration, amount of extraction solvent added, extractive time and times of extraction was used as the 4 influencing factors. Results The optimum condition was the extraction in 80% ethanol, two times and 1 h. Conclusion The optimal extracting method of the total saponins of M. nepalensis is suitable and feasible by uniform design. The study could provide scientific references for further investigation of M. nepalensis.
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[基金项目]
四川省科技厅应用基础研究项目(2009JY0017);西南民族大学中央高校基本科研业务费专项资金资助(09NZYZJ01)