目的 对大孔树脂富集纯化广金钱草总黄酮的工艺条件进行初步探索。方法 采用紫外-可见分光光度法，以总黄酮吸附率、解吸率为评价指标，通过考察树脂类型、原料液质量浓度、pH值、上样体积流量、解吸溶剂、水除杂用量、最佳上样量、醇洗脱体积流量、洗脱体积及绘制树脂静态吸附平衡曲线、泄露曲线和动态解吸曲线，综合评判确定最优工艺。结果 DM-130树脂富集纯化效果较好，其对广金钱草总黄酮的适宜吸附和解吸条件为：吸附原料液质量浓度为8.0 mg/mL，pH值为4.0～6.0，上样体积流量为3 BV/h；解吸先用4 BV的水除杂，再用50%乙醇洗脱，洗脱剂用量为5 BV，体积流量为2.0 BV/h。结论 DM-130树脂可用于广金钱草总黄酮的富集纯化，该方法具有操作简单、成本低廉、精制效果突出等特点，具有较高的工业生产应用价值。

Objective To primarily study the enrichment and purification technology of total flavonoids from Desmodium styracifolium with macroporous resin. Methods Taking the adsorption rate and desorption rate as the appraisal target, the ultraviolet-visible spectrophotometry was applied for studying the optimal enrichment technique through evaluating the impact of resin type, original concentration, pH value, flow rate, desorption solvent, water used for purification, the optimal sample amount, flow rate, solvent volume, static adsorption equilibrium curve, overload curve, and dynamic desorption curve. Results It was proved that DM-130 resin could effectively enrich the total flavonoids from D. styracifolium. The preferred condition for the procedure was as following: original concentration of 8.0 mg/mL, pH value of 4.0—6.0, flow rate for sampling of 3 BV/h, removing impurities by 4 BV of water and then eluting the total flavonoids with 50% ethanol for 5 BV at flow rate of 2.0 BV/h. Conclusion DM-130 resin can be used for purification and enrichment of total flavonoids from D. styracifolium, which has the characteristics of easy operation, low cost, highlight purification effect, and high industrial production value.