[关键词]
[摘要]
目的 考察生地黄不同炮制阶段所含地黄寡糖和梓醇量的变化。方法 采用水为溶媒,超声提取不同炮制阶段生地黄中寡糖类成分,流动相用乙腈-水(72∶28),体积流量1.0 mL/min,NH2色谱柱分离,示差折光检测器检测,HPLC法测定;采用甲醇为溶媒,超声提取梓醇,流动相用甲醇-0.1%磷酸溶液(1∶99),体积流量1.0 mL/min,C18色谱柱分离,检测波长210 nm,HPLC法测定。结果 不同炮制阶段生地黄中的寡糖成分及其量不同,梓醇的量不同。结论 炮制改变了生地黄中寡糖成分类型及其量,同时也影响梓醇的量。
[Key word]
[Abstract]
Objective To investigate the contents of oligosaccharide and catalpol in Rehmanniae Radix at various processing phases. Methods Saccharide ingredient was extracted by ultrosonic extraction when water was used as solvent, separated by NH2 chromatographic column while acetonitrile-water (72∶28) as mobile phase at flow rate of 1.0 mL/min, detected by differential refractive detector, and assayed by HPLC. Catalpinol was extracted by ultrosonic extraction when methanol was used as solvent, separated by C18 chromatographic column while methanol-0.1% phosphoric acid (1∶99)as mobile phase at flow rate of 1.0 mL/min, detected at wavelength of 210 nm, and assayed by HPLC. Results There are significantdifferences at various processing phases of oligosaccharide and catalpol contents in Rehmanniae Radix. Conclusion Processing could change the content and composition pattern of oligosaccharide in Rehmanniae Radix and affect the content of catalpol in Rehmanniae Radix at the same time.
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[基金项目]
国家自然科学基金资助项目(30672643,30472186,30772773);甘肃省自然科学基金资助项目(3ZS051-A25-078)