目的 探讨绶草内生真菌与其宿主化学成分的相关性,为阐明药用植物真菌与宿主植物产生相同或相似化学成分的机制奠定基础。方法 采用HPLC法对绶草及其内生真菌各提取部位进行色谱分析,并利用SPSS16.0软件对色谱结果进行聚类,得到绶草内生真菌之间化学成分的相关性。结果 绶草内生真菌石油醚提取物的聚类规律不明显;醋酸乙酯提取物及水饱和正丁醇提取物均表现为分离自同一部位的真菌倾向于聚类在同一分支,相同种属的内生真菌同样倾向于聚类在同一分支,且以醋酸乙酯提取物表现最为明显。结论 绶草内生真菌石油醚提取物之间的相关性最小,醋酸乙酯提取物与真菌分离部位相关性最为明显,与真菌的种属同样存在较大的相关性。

Objective To discuss correlations between the chemical constituents of different endophytic fungi and their host and provide basis of researching the mechanism of some endophytic fungi which have the abilities to produce the same or similar chemical constituents as their host plant product. Methods Chemical compounds derived from the endophytic fungi and their host Spiramthes sinensis were tested by HPLC, and the correlation data were analysed by SPSS16.0. Results The clustering is not obvious between petroleum ether extracts of fungi; the result of study on the ethyl acetate extracts and water-saturated n-butanol extracts showed that fungi isolated from the same organ tended to cluster together and the same species fungi tended to cluster together. Conclusion Correlation among the petroleum ether extracts of the fungi is not obvious. The correlation between the ethyl acetate extracts are the most obvious in organ from which the fungi isolated and within the species.

国家自然科学基金面上项目(30970300);黑龙江中医药大学校重点基金资助项目(200506);黑龙江省博士后基金资助项目(LBH-Z08008)