目的探讨白藜芦醇对人外周血γδT细胞生长和杀伤功能的影响。方法用异戊烯焦磷酸法体外扩增人γδT细胞,不同浓度的白藜芦醇作用γδT细胞后,MTT法检测γδT细胞生长情况,流式细胞术检测给药前后γδT细胞杀伤功能相关标志物颗粒酶B、穿孔素和CD107a的表达。结果人外周血单核细胞(PBMC)培养10 d后,γδT细胞比例从扩增前的3.12%增至80.46%;白藜芦醇浓度为0.2～12.5μmol/L时能促进γδT细胞生长,其中以1.56μmol/L作用最明显,使细胞增殖率高出对照组约15.90%;白藜芦醇1.56μmol/L可上调γδT细胞杀伤功能相关标志物颗粒酶B、穿孔素、CD107a表达,随着给药浓度增高,γδT增殖及其颗粒酶B、穿孔素、CD107a表达逐渐降低。结论白藜芦醇在一定浓度范围内可促进γδT细胞增殖,且增强γδT细胞杀伤功能相关标志的表达,这可能是其抗肿瘤作用机制之一。

Objective To investigate the effect of resveratrol on hunman γδT cell growth and killing function. Methods Using the isopentenylpyrophosphate medthod to cultivate human peripheral blood γδT cell in vitro. Effect of resveratrol on γδT cell growth at different concentrations was observed by MTT, flow cytometry was used to detect the γδT cell granzyme B, perforin, and CD107a related to killing function before and after administration. Results γδT cells were amplified from 3.12% to 80.46% after human peripheral blood mononuclear cells (PBMC) being cultured for 10 d. The certain concentration (0.2－12.50 μmol/L) of resveratrol could promote the growth of γδT cell. At the concentration of 1.56 μmol/L, resveratrol could promote γδT cells most significantly with proliferation rate 15.90% higher than that in the control group, and at this concentration resveratrol could significantly up-regulate the expression of granzyme B, perforin, and CD107a related to killing function of γδT cells. With the increasing concentration of resveratrol, the proliferation of γδT cells and the expression of granzyme B, perforin, and CD107a were reduced. Conclusion Resveratrol at certain concentration could promote γδT cell proliferation and enhance the expression of its related markers with killing function, which is one of the antitumor mechanisms.

南京军区科技创新经费资助项目(08MA039)