[关键词]
[摘要]
目的寻找一种有效富集苦参总黄酮的方法并对工艺条件进行初步探索。方法样品溶液以聚酰胺吸附,水洗除杂,将除杂后的聚酰胺加于大孔吸附树脂顶部,以一定体积分数的乙醇溶液洗脱富集总黄酮。结果聚酰胺与AB-8大孔树脂联用能有效富集总黄酮,且可与其生物碱类成分有效分离。最佳工艺条件为浸膏与聚酰胺比为1∶1,与树脂比为1∶3,以80%乙醇洗脱,收集洗脱液3 BV,富集总黄酮质量分数(53.6±2.2)%,收率为(72.1±3.7)%。结论该方法优于目前常用的黄酮富集方法,且工艺简单,具有良好的应用前景。
[Key word]
[Abstract]
Objective To explore the enriching method for the total flavonoids from Sophora flavescens with polyamide-macroporous resin. Methods Polyamide adsorbed with sample was washed with water first to eliminate the impurities and then was loaded on the top of macroporous resin. The total flavonoids were enriched by eluting with ethanol. Results AB-8 resin combined with polyamide could effectively enrich the total flavonoids and separate them thoroughly from the alkaloid compounds. The preferred condition for the procedure was as following: the weight of polyamide and dry AB-8 resin was one and three times of the extract weight (1:1 and 1:3), respectively. The volume of 80% ethanol for elution was 3 times of the column volume. The purity of total flavonoids reached to (53.6 ± 2.2)% and the recovery rate was (72.1 ± 3.7)%. Conclusion The method is not only superior to the others for enriching total flavonoids but also more convenient, which has a good application foreground.
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[基金项目]
国家自然科学基金资助项目(30901953);国家教育部新教师基金项目(20094425120001);“重大新药创制”国家科技重大专项(2009ZX09304-003,2009ZX09501-017)