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[摘要]
目的 研究裂蹄木层孔菌的抗肿瘤作用及其机制。方法 制备S180、H22、MFC实体瘤小鼠模型和S180腹水瘤小鼠模型。将3种实体瘤模型小鼠均分为模型组,裂蹄木层孔菌低、中、高剂量(0.5、1.0、2.0 g/kg)组和环磷酰胺(CTX)阳性对照组,S180腹水瘤小鼠模型加设对照组。观察裂蹄木层孔菌对腹水瘤小鼠生存时间以及实体瘤小鼠瘤质量、胸腺和脾脏指数、血清中白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)水平的影响。结果 与模型组比较,裂蹄木层孔菌低、中、高剂量均能延长S180腹水瘤小鼠的生存时间(P<0.05),抑制肿瘤生长(P<0.05),提高胸腺和脾脏指数(P<0.05)及血清IL-2(P<0.05)和TNF-α水平(P<0.01)。结论 裂蹄木层孔菌对小鼠肉瘤S180、肝癌H22细胞、前胃癌MFC肿瘤的生长有明显抑制作用,显示一定的抗肿瘤效应,其可能通过提高免疫器官指数及血清中IL-2、TNF-α的水平达到抗肿瘤目的。
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[Abstract]
Objective To study the antitumor effect of Phellinus linteus and preliminarily discuss its mechanism. Methods MFC-bearing, H22-bearing, S180-bearing, and S180 ascites tumors mice models were established. Five groups, model group, cyclophosphamide (CTX) group, high- (2 g/kg), middle- (1 g/kg) and low-dose (0.5 g/kg) of P. linteus groups, were studied for the former three models and a normal control group was added for S180 ascites tumors mice model. Then the life span prolongation of ascites tumor mice and tumor weight, thymus and spleen indexes, and interleukin-2 (IL-2) and tumor necrosis factor (TNF-α) levels of S180-bearing mice were determined. Results Compared with model group, high-, middle-, and low-dose groups of P. linteus could prolong life span (P<0.05), inhibit tumor growth (P<0.05), increase thymus and spleen indexes (P<0.05), and elevate IL-2 and TNF-α levels (P<0.01) of ascites tumors mice. Conclusion P. linteus shows antitumor effects and can obviously inhibit the cell growth of MFC, H22 and S180 through increasing index of immune organ and the IL-2 and TNF-α levels of serum.
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