目的探讨虫草复方对博莱霉素致大鼠肺纤维化的作用及其机制。方法 Wistar大鼠随机分为对照组,模型组,复方虫草高、低剂量(150、75 mg/kg)组和醋酸泼尼松(3.33 mg/kg)组,每组18只。除对照外其余各组大鼠采用气管内一次性滴注盐酸博莱霉素造模,对照组大鼠气管内一次性滴注等体积生理盐水。造模后第2天开始给药,对照组和模型组分别ig给予等体积生理盐水。各组动物于7、14、28 d随机处死6只,分别观察大鼠肺指数、肺组织羟脯氨酸(Hyp)、丙二醛(MDA)和超氧化物歧化酶(SOD)活性及血清中MDA的量和SOD的活性。同时取固定部位肺组织进行病理组织学检查,并应用免疫组织化学法检测转化生长因子-β1(TGF-β1)、Ⅰ型胶原、Ⅲ型胶原在肺组织中的表达;观察各组大鼠动脉血气的变化。结果虫草复方能明显降低肺纤维化大鼠肺指数和肺组织Hyp的量,并可提高血清和肺组织中SOD活性,降低血清和肺组织中MDA和肺组织TGF-β1、Ⅰ型胶原、Ⅲ型胶原的表达;虫草复方可使肺纤维化大鼠动脉二氧化碳分压[p(CO₂)]降低和血氧分压[p(O₂)]提高;虫草复方明显改善实验性大鼠肺纤维化程度。结论虫草复方对大鼠肺纤维化具有一定的干预作用,其机制可能与抗脂质过氧化、抑制TGF-β1表达、减少肺泡间质胶原沉积及改善肺呼吸功能有关。

Objective To study the effect of Cordyceps sinensis and Panax notoginseng compound extracts(CSPNE) on Bleomycin-induced pulmonary fibrosis(PF) in rats and its mechanisms.Methods Wistar rats were randomly divided into five groups(18 rats in each group).Rats in model,high-and low-dosage(150 and 75 mg/kg) CSPNE,and Prednisone acetate(3.33 mg/kg) groups were injected with a single dose of Bleomycin hydrochloride through trachea and in control group with the same volume of normal saline.One day after the injection,CSPNE solution of different dosages was respectively ig given to rats daily,while the same volume of normal saline was given to those in the control and model groups.On day 7,14,and 28 after medication,six rats in per treatment group were randomly killed.Lung samples in every group of rats were measured for lung index,hydroxyproline(Hyp) and malonaldehyde(MDA) contents,and superoxide dismutase(SOD) activities,and serum was analyzed for SOD activities and MDA contents.Lungs were incised to make pathological sections which were stained with HE,and the expression of TGF-β1,Collagen I,and Collagen III in lung tissue was analyzed by means of immunohistochemistry technique.Arterial blood gas detection was observed in every group.Results CSPNE could significantly decrease the lung index and Hyp contents in the lung tissue of rats with PF.It could enhance SOD activities and reduce MDA contents both in serum and lung tissues.It also decreased the expression of TGF-β1,Collagen I,and Collagen III in lung tissues.In the arterial blood gas detection CSPNE was found to make [p(CO2)] lowered,while [p(O2)] increased in the artery of rats with PF.Conclusion CSPNE has obvious effect of inhibiting PE of rats,and the mechanism may be associated with its antilipid peroxidation,inhibiting the expression of TGF-β1,reducing the alveolar interstitial collagen deposition,and improving respiratory function as well.

香港保健协会项目(20040903,HK)