[关键词]
[摘要]
采用连续泡沫分离法纯化无患子总皂苷,确定泡沫分离最佳工艺条件。方法采用正交试验法与分光光度法,以无患子总皂苷富集比、收率和质量分数为评价指标,确定泡沫分离的最佳工艺条件。结果连续泡沫法分离无患子总皂苷的最佳工艺条件为:进料液中无患子总皂苷质量浓度为20 mg/L,pH值为4.40,进料口高度为40 cm,气体体积流量为0.6 L/min,进料液体积流量为35 mL/min;通过连续泡沫分离法纯化得到无患子总皂苷,收率为23.64%、质量分数为90.27%。结论连续式泡沫分离法纯化无患子总皂苷具有操作简单、能耗低等优点,可工业化应用。
[Key word]
[Abstract]
Objective The continuous foam separation was adopted to optimize the process conditions for separation of tatal saponins in Sapindus mukorossi.Methods Orthogonal test and spectrophotometry were employed to determine the optimum conditions of foam separation using the enrichment ratio,yield,and purity of total saponins in S.mukorossi as evaluating indexes.Results The best condition of the process was as follows: gas flow rate of 0.6 L/min,feed height of 40 cm,liquid flow rate of 35 mL/min with initial feed total saponins in S.mukorossi at concentration of 20 mg/L with pH 4.40.Conclusion The yield and purity of the isolated total saponins in S.mukorossi were 23.64% and 90.27%,respectively.The continuous foam separation could conduct in relatively simple equipment,low energy consuming and provide the possibility to future industrial applications.
[中图分类号]
[基金项目]
安徽高校省级自然科学研究重点项目(KJ2009A002);合肥工业大学研究生创新基金项目(XS08042)