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[摘要]
目的 通过测定并比较柱果铁线莲、单叶铁线莲、威灵仙、山木通、毛蕊铁线莲、转子莲、女萎和天台铁线莲的ITS序列,为铁线莲属药用植物的分子鉴定提供依据。方法 利用PCR法从叶片基因组DNA中扩增ITS,借助Clustal X、MEGA、DNASTAR等软件比较和分析ITS的序列特征。结果 铁线莲属8种药用植物ITS1与ITS2之间的长度为534~561 bp,变异位点50个,信息位点22个;天台铁线莲与转子莲的遗传距离最小,与女萎遗传距离最大,亲缘关系最远。序列已提交至NCBI数据库,登录号为JF714638-JF714645。结论 获得铁线莲属8种药用植物的ITS序列,为分子鉴定奠定了基础。
[Key word]
[Abstract]
Objective To provide molecular evidence for identification of medicinal plants in Clematis L., sequencing and comparison of ITS from C. uncinata, C. henryi, C. chinensis, C. finetiana, C. lasiandra, C. patens, C. apiifolia, and C. patens ssp. tientaiensis were performed. Methods ITS sequences were amplified from leaf genomic DNA by PCR. Sequence features were compared and analyzed using Clustal X, MEGA, and DNASTAR softwares. Results Between ITS1 and ITS2 of the eight medicinal plants in Clematis L., the length of ITS region varied from 534-561 bp with 50 variable sites and 22 parsimony information sites. The smallest genetic distance was observed between C. patens ssp. tientaiensis and C. patens, and the largest existed between C. patens ssp. tientaiensis and C. apiifolia revealing their farest genetic relationship. Sequences were submitted to NCBI database with the registry numbers of JF714638-JF714645. Conclusion ITS sequences of the eight medicinal plants in Clematis L. are obtained, which could provide a foundation for molecular identification.
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[基金项目]
浙江省新苗人才计划项目(2009R428012)