[关键词]
[摘要]
目的 建立银耳多糖质量控制方法。方法 采用高效凝胶渗透色谱(HPGPC)法测定银耳多糖相对分子质量及其分布和多糖的量。色谱条件:Shodex Sugar KS-804(300 mm×8 mm,7 μm)和KS-805(300 mm×8 mm,17 μm)凝胶排斥色谱柱串联做为分离柱,流动相为水,体积流量0.8 mL/min,柱温40 ℃,进样量40 μL,检测条件为示差检测器。结果 银耳多糖峰出现在14.40~15.30 min,对应相对分子质量为1.26×106~1.39×106。在0.01~2.0 mg/mL呈良好的线性关系(r=0.999)。银耳多糖质量浓度范围分别为:1.1~1.5 mg/mL(以透析银耳多糖为对照)和0.8~1.1 mg/mL(以蓝色葡聚糖为对照)。多糖检测限和定量限分别为0.8 μg和2 μg。结论 所建立的方法简单、快速、准确、重现性好、灵敏度较高,可作为银耳多糖质量控制的方法。
[Key word]
[Abstract]
Objective To develop a high performance gel permeation chromatography (HPGPC) method for quality control of Tremella fuciformis polysaccharide. Methods The separations were carried out with two series-wound columns including Shodex Sugar KS-804 (300 mm × 8 mm, 7 μm) and KS-805 (300 mm × 8 mm, 17 μm), water as mobile phase at flow rate 0.8 mL/min, column temperature at 40 ℃, injection volume 40 μL, and differential index detector. Results T. fuciformis polysaccharide peak was located within 14.40—15.30 min and corresponding molecular weight was 1.26 × 106—1.39 × 106. The linearity was good during 0.01—2.0 mg/mL (r = 0.999). The polysaccharide concentration was 1.1—1.5 mg/mL and 0.8—1.1 mg/mL with precipitation of T. fuciformis polysaccharide and blue dextran as reference, respectively. LOD and LOQ were 0.8 and 2 μg, respectively. Conclusion The method is simple, quick, and accurate, and has good repeatability and high sensitivity, which is fitful for quality control.
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[基金项目]
国家自然科学基金资助项目(30870551)