[关键词]
[摘要]
目的 制备10-羟基喜树碱(HCPT)缓释微胶囊,测定其包封率,并考察其体外释药特性。方法 以生物相容性良好的聚赖氨酸和海藻酸钠为囊壁材料,HCPT药物微粒为囊芯,采用静电吸引层层纳米自组装技术(LBL法)制备HCPT微胶囊。结果 LBL法制备的HCPT微胶囊用扫描电子显微镜和激光共聚焦显微镜观察具有明显的核-壳结构;HCPT微胶囊的包封率较高,达到(68.41±0.72)%;随着包裹层数的增加,HCPT微胶囊在体外释放速率降低。结论 用LBL法制备的HCPT微胶囊具有较高的包封率和缓释特性,具有一定的应用前景。
[Key word]
[Abstract]
Objective To prepare microcapsules for controlled release of 10-hydroxycamptothecin (HCPT), and determine its encapsulation efficiency and drug release profile in vitro. Methods The microcapsules loaded with HCPT were prepared using a novel coating technology based on the layer-by-layer (LBL) deposition of oppositely charged polylysine (PLL) and alginate (ALG) polyelectrolytes with good biocompatibility onto microcrystal templates. Results The microcapsules fabricated by this method had visible core-shell structure observed by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM); The entrapment efficiency of the microcapsules was as high as (68.41 ± 0.72)%; The release rate of HCPT decreased as the bilayers increased in vitro. Conclusion The microcapsules of HCPT prepared by LBL have higher entrapment efficiency and are characterized with controlled drug release, which has potential for future application.
[中图分类号]
[基金项目]
“948”计划资助项目(2011-4-16);黑龙江省留学归国基金资助项目(LC201004)