目的 研究微波辐射对淫羊藿朝藿定B提取的影响。方法 采用单因素试验逐一考察提取溶剂、料液比、微波辐射时间对朝藿定B提取率的影响；采用紫外-可见光分光光度法考察微波辐射对朝藿定B稳定性的影响；采用石蜡切片方法研究微波辐射对淫羊藿材料的影响。结果 乙醇体积分数和微波辐射时间对提取率的影响较大，料液比影响较小。微波辐射能引起叶片组织等的破损，但不会影响朝藿定B的稳定性。结论 微波辐射能够提升提取效果，其机制可能是微波辐射促进了乙醇溶液进入叶片组织和朝藿定B溶出叶片组织的传质过程。

Objective To investigate the influence of microwave radiation on the extraction of epimedin B from Epimedii Folium. Methods The effects of extraction solvent, ratio of material to liquor, and duration of microwave radiation on the extraction yield of epimedin B were studied by single factor tests. The influence of microwave radiation on the stability of epimedin B was assessed by UV-Vis spectrophotometeric analysis. The impacts of microwave radiation on leaf sample were observed using paraffin section method. Results The contributions of ethanol concentration and duration of microwave radiation for the extraction yield of epimedin B are significant, whereas ratio of material to liquor is not a significant factor. Microwave radiation could result in the disruptions of leaf tissues and other parts, but it did not affect the stability of epimedin B. Conclusion Microwave radiation could facilitate the extraction of epimedin B, which might result from the enhancement of the mass transfer of ethanol solution into leaf tissues and dissolution of epimdin B from the matrix.

中央高校基本科研业务费专项资金项目（GK200902043）；大学生创新性实验计划项目（101071817）