目的考察巫山淫羊藿悬浮培养细胞的生长特性,为巫山淫羊藿资源利用提供参考。方法选用悬浮培养所获得的均匀悬浮培养细胞为研究对象,在光照条件下进行悬浮培养,每隔3 d取样,测定相关的生理生化指标。结果巫山淫羊藿悬浮细胞的生长曲线呈"S"型,培养基中蔗糖、磷酸盐、硝酸盐、铵盐量均呈急剧下降趋势,最后变化趋于平缓,细胞合成黄酮最佳光照时间为每天12 h,黄酮合成量为27.854 82 mg/L。结论巫山淫羊藿细胞生长特性的研究为其大规模悬浮培养生产天然活性成分奠定了基础。

To provide biotechnology reference for the utilization of Epimedium wushanense. Methods Some subculture conditions were determined and the growth characteristics of suspension cells were observed every 3 d. Results The cell showed S-shaped growth curve and the content of nitrate, ammonia salt, phosphate, and sucrose all showed sharply decreasing trend during the initial cell suspension culture of E. wushanense. Then the respective changes slowed down. The optimum illumination duration was 12 h. The highest output of flavonoids was 27.854 82 mg/L under 12 h illumination. Conclusion The growth characteristics research lays the foundation of mass natural active ingredient in cell suspension culture production of E. wushanense.

四川省教育厅项目(09ZX011);四川省科技厅应用基础研究项目(2008JY0158);四川省重点实验室开放基金项目(XNYB09-04)