目的研究我国黄花蒿天然群体种质资源的遗传多样性,并对其进行SRAP分析。方法采用SRAP分子标记技术,以我国天然群体主要分布区域的60份黄花蒿种质资源为试验材料,运用Popgene version 1.31软件和Treeconw软件计算相关参数,UPGMA方法聚类,构建亲缘关系树状图。结果 24对SRAP引物组合扩增出232条带,多态性比率(PPB)为81.47%。SRAP标记的Nei’s基因多样性(H)为0.190 5,Shannon’s信息指数(I)为0.300 0。青蒿素量高的4个地区之间,多态位点百分率在50.00%～61.21%,平均为55.82%,SRAP标记的H值为0.155 7～0.179 3,I值为0.237 9～0.276 6;SRAP检测不同材料间遗传相似系数为0.643 2～0.872 7,平均为0.754 7。结论 SRAP标记能有效地揭示我国野生黄花蒿丰富的遗传多样性,为黄花蒿新品种选育提供了物质基础。

The genetic diversity of Artemisia annua germplasmic resources in natural populations of China was analyzed by SRAP marker. Methods Taking 60 germplasm of A. annua version in main distribution areas of natural population in China as testing materials, the relative parameters were calculated by Popgene version 1.31 and Treeconw software. The systematic diagram of genetic relationship was made up by Treeconw software and clustered by UPGMA method. Results The 232 DNA bands were amplified with 24 pairs of SRAP primer combinations, and the percentage of polymorphic bands (PPB) was 81.47%. At species level: Nei’s gene diversity (H) was 0.190 5 using SRAP marker. Shannon’s information index (I) was 0.300 0 by SRAPs; Among the four areas with higher level of artemisinin: PPB were 50.00%—61.21%, the average was 55.82%. He were 0.155 7-0.179 3 by SRAP marker. I were 0.237 9—0.276 6 by SRAP maker; Genetic distance were 0.643 2-0.872 7, the average was 0.754 7. Conclusion SRAP marker is an efficient method in revealing the abundant genetic diversity of wild A. annua in China, which provides the material basis for promising germplasm of further breeding.

国家科技攻关计划(2004BA721A32);国家科技支撑计划(2006BAI09B01-04,2006BAI09B02-04);国家中医药管理局行业专项(201107011)