[关键词]
[摘要]
目的 研究黑乳海参Holothuria nobilis的化学成分。方法 应用多种色谱技术对黑乳海参的化学成分进行分离纯化,根据化合物的理化性质和波谱数据鉴定结构。结果 分离得到了2个三萜皂苷元类化合物,应用化学方法和各种光谱技术鉴定其结构为3-O-{3''''-O-甲基-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃葡萄糖-(1→4)-β-D-吡喃奎诺糖-(1→2)-4′-O-磺酸钠-β-D-吡喃木糖}-海参烷-22, 25-环氧-9-烯-3β, 12α, 17α, 25β-四醇(1)和3-O-{β-D-吡喃奎诺糖-(1→2)-4′-O-磺酸钠-β-D-吡喃木糖}-海参烷- 9-烯-18, 16-内酯环-22, 25环氧-3β, 12α, 17α-三醇(2)。结论 2个化合物均为新化合物,命名为黑乳海参苷I(nobiliside I,1)和黑乳海参苷II(nobiliside II,2)。
[Key word]
[Abstract]
Objective To study the constituents from the sea cucumber Holothuria nobilis. Methods The compounds of the sea cucumber were separated by multi-chromatography and their structures were elucidated by physico-chemical and spectral analyses. Results Two new triterpene glycosides were isolated and their structures have been deduced by extensive spectral analysis (IR, 2D-NMR, and ESI-MS) and chemical structures as 3-O-{3′′′′-O-methyl-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D- quinovopyranosyl-(1→2)-4′-O-sulfate-β-D-xylopyranosyl}-holosta-22, 25-epoxy-9-ene-3β, 12α, 17α, 25β-tetraol-sodium salt (nobiliside I, 1) and 3-O-{β-D-quinovopyranosyl-(1→2)-4′-O-sulfate-β-D-xylopyranosyl}-holoshillaside 18 (16)-lactone-22, 25-epoxy-9-ene-3β, 12α, 17α-triol sodium (nobiliside II, 2). Conclusion Two aglycons are both new compounds named nobiliside I and nobiliside II.
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[基金项目]
浙江省自然科学基金资助项目(Y2110082);浙江省科技厅公益项目(2010C33136);宁波市自然科学基金资助项目(2007A610083, 2010A610020);浙江省医药卫生科学研究基金资助项目(2008B163);浙江省高校科研基金资助项目(Y200805910)