目的采用复合酶-微波法从绿茶中提取茶多酚。方法通过单因素试验法和正交试验法确定复合酶-微波法提取茶多酚的最佳工艺,同时比较复合酶-微波法、复合酶法、索氏法和微波法对提取效果的影响。结果优化得到提取茶多酚的最佳工艺为:在50℃条件下用纤维素酶和果胶酶前处理40 min,微波辐射8 min,微波功率500 W,料液比1:30,25%乙醇溶液作为萃取剂。结论验证试验表明,最佳工艺可行;从提取时间和提取效率来看,复合酶-微波法提取茶多酚比复合酶法、索氏法和微波法耗时少、提取效率高。

The feasibility of employing complex enzyme-microwave-assisted extraction (EMAE) of tea polyphenols from green tea was demonstrated. Methods The optimal extraction parameters based on complex EMAE technique were optimized by orthogonal test and single factor test. The effects of complex EMAE, complex enzyme, Soxhlet method, and microwave method on extraction were compared simultaneously. Results The optimal extraction conditions were as follows: the pre-leaching time of 40min using cellulase and pectinase enzyme at 50 ℃, the radiation time of 8 min; microwave power of 500 W, solid/liquid ratio of 1:30;and 25% ethanol aqueous solution. Conclusion Validation tests showed that the best technology was feasible, from the points of extraction time and the extraction efficiency, complex EMAE method is more effective and rapid than Soxhlet extraction, microwave-assisted, and complex enzyme methods.

江西省教育厅重点项目(GJJ08200)