目的 评价清开灵和双黄连口服液体内抗禽流感病毒药效，探讨其对感染流感病毒小鼠免疫功能的影响。方法 建立H9N2亚型禽流感病毒鼠肺适应株人工感染BALB/c小鼠模型，以肺指数抑制率、生命保护率和肺病毒滴度为主要评价指标，研究清开灵和双黄连口服液对感染H9N2亚型禽流感病毒小鼠的防治效果；以脾指数、胸腺指数和T细胞亚群比率（CD4⁺/CD8⁺）为主要评价指标，探讨清开灵和双黄连口服液对感染流感病毒小鼠免疫功能的影响。结果 清开灵和双黄连口服液均能显著抑制H9N2亚型禽流感病毒引起的小鼠肺炎实变，攻毒后第4天小鼠肺指数抑制率分别为34.1%、26.3%。清开灵和双黄连口服液对感染小鼠有显著的生命保护作用，存活率分别为70.0%、60.0%，显著高于病毒对照组的存活率（30.0%），鼠肺病毒滴度显著低于病毒组（P＜0.01）。清开灵和双黄连口服液对感染病毒后小鼠脾脏和胸腺萎缩具有显著的抑制作用，并能提升感染小鼠脾脏中CD4⁺/CD8⁺值。结论 清开灵和双黄连口服液具有显著的抗禽流感病毒作用，对病毒复制的抑制及对病毒感染导致的小鼠免疫功能下降的调节作用是其发挥抗病毒功效的重要机制。

Objective To evaluate the activity of Qingkailing and Shuanghuanglian Orals against avian influenza virus in vivo, and investigate the effect of the two Chinese medicinal formulas on immunologic function of infected mice. Methods The BALB/c mouse model with viral pneumonia by avian influenza H9N2 virus was adopted. Protective effects of Qingkailing and Shuanghuanglian Orals against H9N2 virus in mice were investigated based on the lung index inhibitory rate, life-protection rate, and virus titer in lungs. Furthermore, the immunologic functions of the two Chinese medicinal formulas on infected mice were investigated based on the spleen index, the thymus index, and the percentage of T cell subgroups (CD4⁺/CD8⁺). Results Qingkailing Oral, as well as Shuanghuanglian Oral, significantly inhibited the lung consolidation, improved the survival rate, and reduced the virus titer on mice infected with H9N2 virus. The lung index inhibitory rates were 34.1% and 26.3%, and the life-protection rates were 70.0% and 60.0%, respectively. Whereas, life-protection rate of the viral control group was 30.0%. Qingkailing and Shuanghuanglian Orals significantly exhibited the prevention against atrophy of spleen and thymus of mice caused by viral infection, and improved the percentage of CD4⁺/CD8⁺ T cells in spleen of infected mice. Conclusion Qingkailing and Shuanghuanglian Orals have ideal efficacy against influenza virus in vivo. Inhibition on viral multiplication and adjustment for deviant immunologic function of infected mice are verified as the mechanism of action of the two Chinese medicinal formulae against influenza virus.

国家自然科学基金项目（30972217）；广东省教育部产学研结合项目（2009B090300331）；广东省科技计划重点项目（2009B020600001）