目的 利用色谱法分离与鉴定椿根皮Ailanthus altissima的化学成分。方法 采用乙醇提取法对椿根皮进行回流提取，应用制备液相色谱法对椿根皮粗提物进行分离纯化，分离化合物的结构由质谱和核磁共振氢谱、碳谱鉴定。结果 分离纯化得到臭椿苦酮（1）、臭椿辛内酯A（2）和一新化合物铁屎米酮糖酯（3），且质量分数均在95%以上，化合物3为一个新的β-卡波啉生物碱。结论 该法可以快速、准确地对椿根皮中含量较低的化合物进行提取与鉴定。

Objective Isolation and identification of chemical components from the root bark of Ailanthus altissima by HPLC method. Methods Crude products which were extracted with ethanol were separated and purified by preparative HPLC, and the structures of the compounds were identified by mass spectrometry (MS), ¹H-NMR, and ¹³C-NMR. Results Under the above conditions, ailanthone (1), shinjulactone A (2), and a new β-carboline alkaloid (3) were successfully obtained, and the purities of the compounds are all above 95%. Conclusion A rapid and simple method is set up to isolate and identify lower content components from A. altissima.

大连市科技局科技计划项目（2008E11SF162）