目的 优化酶法辅助提取绞股蓝中总黄酮工艺。方法 采用响应面法优化工艺,绞股蓝经纤维素酶处理后用95%乙醇提取总黄酮,研究酶用量、酶解温度、酶解时间和酶解pH值对总黄酮得率的影响。结果 酶法提取的最佳工艺条件:酶用量120 U/mL,酶解温度49℃,酶解时间150 min,酶解pH值3.9,总黄酮得率可达0.357%。结论 纤维素酶辅助提取绞股蓝中总黄酮工艺稳定可行。

Objective To optimize the extracting process of total flavonoids from Gynostemma pentaphyllum. Methods The total flavonoids were extracted with ethanol 95% after the cellulose treatment to G. pentaphyllum, and the effects of enzyme dosage, enzymatic treatment temperature, enzymatic treatment time, and pH value on the total flavonoids yield was studied with response surface method. Results The optimal enzymatic extracting process of total flavonoids was as follows: enzyme dosage 120 U/mL, enzymatic temperature 49 ℃, enzymatic time 150 min, and pH value 3.9, and the total flavonoids yield reached 0.357%. Conclusion Cellulase is suitable for the total flavonoid extraction from G. pentaphyllum.

湖南省科技计划项目(2009FJ3028)