目的 观察杭白菊、当归、丹参提取液对im黄体酮及紫外（UV）照射导致的黄褐斑小鼠模型的抗氧化与抗黄褐斑形成作用。方法 将小鼠随机分成对照组、模型组、维生素C组、杭白菊组、当归组、丹参组共6组。采用im黄体酮及辅助UV照射方法建立黄褐斑小鼠模型。测定各组肝脏及皮肤组织中的谷胱甘肽过氧化酶（GSH-Px）、超氧化物歧化酶（SOD）、酪氨酸酶（TYR）活性与总抗氧化能力（T-AOC），以及丙二醛（MDA）、一氧化氮（NO）、肝脏脂褐质水平，并测定血液流变学指标，进行皮肤病理学检查。结果 杭白菊、当归、丹参提取液显著增强黄褐斑小鼠肝脏和皮肤中GSH-Px、SOD活性，降低MDA水平，抑制TYR增加，使黑素细胞（MC）生成减少，抑制黑色素合成，从而减轻皮肤色素沉着。结论 杭白菊、当归、丹参提取液有一定的防治黄褐斑的功效，其作用与提高机体抗氧化能力，抑制TYR活性，减少黑色素的合成以及改善机体的血液流变学有关。

Objective To investigate the oxidation resistance and removing chloasma function of the extract from Hangzhou Chrysanthemum Flos, Angelicae Sinensis Radix, and Salviae Mmiltiorrhizae Radix et Rhizoma in cholasma model induced by progesterone and ultraviolet radiation. Methods The cholasma model was establised with progesterone (20 mg/kg) by im injection or ultraviolet radiation in female mice. The effects of each group on GSH-Px, SOD, tyrosinase (TYR), MDA, NO, T-AOC, and lipofuscin in skin and liver were detected, and hemorheology and dermatopathology were observed. Results The result showed that the groups with the extract from Hangzhou Chrysanthemum Flos, Angelicae Sinensis Radix, and Salviae Mmiltiorrhizae Radix et Rhizoma could signifcantly increase the activity of GSH-Px and SOD and decrease the contents of MDA and TYR in skin and liver. Such effect conduced that a decrease of melanin production in the nude mice model was observed. Conclusion All the extracts from Hangzhou Chrysanthemum Flos, Angelicae Sinensis Radix, and Salviae Mmiltiorrhizae Radix et Rhizoma have the effect of removing chloasma to various degrees, and the mechanism may be related to enhancing oxidation resistance, deccreasing TYR activity, inhibiting melanocyte proliferation, and improving hemorheology.

粤港关键领域重点突破项目“华南创新中药研究开发与技术服务中心（平台）”（2006A35003001）