目的 研究不同配比当归-红花药物组合的物质基础与效应的相关性，探索两味中药配伍应用的变化规律。方法 利用中医方剂数据库归纳分析当归-红花配伍比例，并以此设计方案，采用HPLC法分析不同配比当归-红花共煎液与单味药材煎液中阿魏酸、羟基红花黄色素A、绿原酸、香豆酸量的变化。采用凝血时间和清除1，1-二苯基-2-三硝基苯肼（DPPH）自由基药效指标评价当归-红花配伍的药理效应。结果 获得应用频率前10位的当归-红花不同配比；HPLC分析结果显示，当归配伍红花有利于有效成分的溶出；与同等生药量的单味药相比，当归配伍红花能延长凝血时间，增加DPPH自由基清除率。结论 当归配伍红花具有合理性，有利于两味药材中芳香酸类成分的溶出，这与配伍后凝血时间延长、DPPH自由基清除率增加具有一定的相关性。

Objective To study the correlation of combinations in different proportions of Angelicae Sinensis Radix and Carthami Flos and explore the regularity in proportion of the two drugs compatibility. Methods Data mining technology was applied to screening the proportions of Angelicae Sinensis Radix and Carthami Flos. The changes of the contents of ferulic acid, hydroxysafflor yellow A, chlorogenic acid, and coumaric acid in the single and mixed decoction of Angelicae Sinensis Radix and Carthami Flos were determined by HPLC. The experiments of thrombin time and scavenging DPPH were carried out to evaluate the pharmacological activities of compatibility between Angelicae Sinensis Radix and Carthami Flos. Results The top 10 proportions of Angelicae Sinensis Radix and Carthami Flos were screening. The contents of ferulic acid, hydroxysafflor yellow A, chlorogenic acid, and coumaric acid were higher than the single decoction of Angelicae Sinensis Radix and Carthami Flos. Thrombin time was prolonged and the activity of scavenging DPPH was enhanced compared to the same drug dosage of the single decoction of Angelicae Sinensis Radix and Carthami Flos. Conclusion Mixed decoction of Angelicae Sinensis Radix and Carthami Flos is rational and good for the stripping of aromatic acids from the drugs, which has some correlation with the change of activities of the compatibility between Angelicae Sinensis Radix and Carthami Flos.

江苏省高校自然基金重大基础研究项目（06KJA36022）；国家教育部“新世纪优秀人才支持计划”（NCET-09-0163）；江苏高等学校优秀科技创新团队支持计划；江苏省方剂高技术研究重点实验室建设项目资助（BM2010576）