[关键词]
[摘要]
目的 建立高效毛细管电泳(HPCE)法同时测定蒙药小白蒿不同加工品中5种黄酮类成分的方法。方法 小白蒿经不同加工工艺处理后,用HPCE法分别测定其中5种黄酮类成分,并对其进行比较。结果 回归方程分别为5,7,3′-三羟基-4′-甲氧基黄酮Y=0.114 3 X+0.032 7,r=0.999 8;5,3′-二羟基-6,7,4′-三甲氧基黄酮Y=0.100 9 X+0.048 5,r=0.999 6;槲皮素Y=0.055 5 X+0.026 8,r=0.999 6;5,7,3′-三羟基-6,4′-二甲氧基黄酮Y=0.113 2 X-0.016 1,r=0.999 3;木犀草素Y=0.097 9 X-0.029 5,r=0.999 4。5种黄酮类成分分别在1.00~40.00、10.00~200.00、5.00~100.00、1.00~40.00、1.00~40.00 μg/mL线性关系良好。平均回收率分别为98.44%、97.75%、97.73%、97.98%、98.07%;RSD分别为1.60%、1.03%、1.57%、0.94%、1.20%。结论 不同加工工艺对小白蒿中5种黄酮类成分有一定影响;以5种黄酮类成分为分析指标,小白蒿最佳加工工艺为60 ℃加热干燥。
[Key word]
[Abstract]
Objective Determining the content of five flavonoids in Artemisia frigida by high performance capillary electrophoresis (HPCE) method to provide reference data for the evaluation of processing products of A. frigida. Methods Treated by different processing technologies, the content of five flavonoids compounds can be determined and compared by HPCE. Results Regression equations were 5,7,3′-triterhydroxy-4′-methoxyflavone Y = 0.114 3 X + 0.032 7, r = 0.999 8; 5,3′- dihydroxy-6,7,4′-tritermethoxyflavone Y = 0.100 9 X + 0.048 5, r = 0.999 6; quercetin Y = 0.055 5 X + 0.026 8, r = 0.999 6; 5,7,3′-triterhydroxy-6,4′-dimethoxyflavone Y = 0.113 2X-0.016 1, r = 0.999 3; luteolin Y = 0.097 9X-0.029 5, r = 0.999 4. Five kinds of flavonoids were good linear relationship at 1.00—40.00, 10.00—200.00, 5.00—100.00, 1.00—40.00, 1.00—40.00 μg/mL. The average recoveries were 98.44%, 97.75%, 97.73%, 97.98%, and 98.07%. And RSD were 1.60%, 1.03%, 1.57%, 0.94%, and 1.20%. Conclusion The results reveal that the different processing technologies have different effects on the content determination of five flavonoids compounds in A. frigida. Using the five flavonoids as testing indexes, the best processing technology of A. frigida is heating and drying to constant weight at 60 ℃.
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[基金项目]
国家自然科学基金资助项目(30960525)