目的 通过对三七种子后熟过程中内源激素变化规律的研究,探索内源激素在种胚发育进程和休眠解除中的作用机制,为三七种子的处理和种源保存奠定基础。方法 采用酶联免疫法(ELISA)对三七种子后熟过程中的内源激素赤霉素(GA)、吲哚乙酸(IAA)、玉米素核苷(ZR)和脱落酸(ABA)进行动态测定。结果 三七种子后熟过程中,内源GA、IAA和ZR的量随种胚的发育进程而增加,而ABA的量则减少。结论 激素平衡的变化是解除三七种子休眠、促进萌发的决定因素之一。

Objective The studies were carried out on the endogenous phytohormone changes of Panax notoginseng seeds during the after-ripening process in order to understand the machanism of endogenous phytohormones (GA, IAA, ZR, and ABA) in the process of embryo development and dormancy release. Methods Dynamic changes of endogenous phytohormones ofP notoginseng seeds were measured by ELISA method through the entire after-ripening process. Results The contents of endogenous GA, IAA, and ZR increased with the embryo development, while the ABA content decreased contrarily. Conclusion The change of phytohormone balance is one of determining factors for relieving dormancy and promoting germination of P. notoginseng seeds.

国家自然科学基金资助项目(30760116);云南省自然科学基金资助项目(2007C204M)