[关键词]
[摘要]
目的 筛选分离楤木总皂苷的最佳树脂,并对影响分离的各种因素进行系统研究,使分离工艺达到最优化。方法 采用静态与动态的吸附-解吸两种方法,以高效液相色谱法测定楤木皂苷A的量为评价指标,进行工艺筛选。结果 AB-8分离效果最好,其最佳工艺为药液质量浓度0.1 g/mL(相当于原生药)、上样量为6 BV(树脂床体积),以2.5 BV/h的吸附速率进行吸附,50%乙醇4 BV、2 BV/h进行洗脱效果最佳。经AB-8处理后的楤木皂苷A收率达30%,楤木总皂苷可达80%以上。结论 该方法简单可行,分离效果好,能满足于大生产的要求。
[Key word]
[Abstract]
Objective To prepare sustained-release honokiol solid lipid nanoparticles (H-SLN). Methods The solid lipid nanoparticles were prepared by emulsion-evaporation-solidified at low temperature method. The optimum condition was performed based on the orthogonal design to examine the entrapment efficiency, the mean diameter of the particles and the release in vitro. Results The entrapment efficiency of honokiol in the nanoparticles was 77.1% and the mean diameter of the particles was 159 nm. Conclusion The emulsion–evaporation- solidified at low temperature method can be used to prepare honokiol solid lipid nanoparticles.
[中图分类号]
[基金项目]
四川省教育厅重点项目(2005A76)