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[摘要]
目的 制备具有缓释作用的和厚朴酚固体脂质纳米粒。方法 采用乳化蒸发-低温固化法制备和厚朴酚固体脂质纳米粒,通过正交试验对处方进行优化,并对其包封率、粒径、体外释放等进行考察。结果 制备的固体脂质纳米粒的平均粒径为159 nm,包封率为77.1%。结论 乳化蒸发-低温固化法可用于制备和厚朴酚固体脂质纳米粒。
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[Abstract]
Objective The aim of the study was to establish a high speed counter-current chromatography (HSCCC) method for the isolation and purification of alpinetin and cardamomin from Alpinia katsumadai. Methods Two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5∶5∶7∶3) was used. The flow rate of the mobile phase was 2.0 mL/min, the revolution speed was 800 r/min, the separation temperature was controlled at 25 ℃, the reservation ratio of the stationary phase was 50%, and the detection wavelength was 300 nm. Results Alpinetin (17.2 mg) and cardamomin (25.1 mg) could be obtained from 100 mg of the crude extract in one-step separation by the method. The purities of them were 98.1% and 99.2%, respectively, as determined by HPLC and their chemical structures were identified by 1H-NMR and 13C-NMR. Conclusion The traditional method, column elution, could not eliminate irreversible adsorption, while the HSCCC method used for the isolation and purification of alpinetin and cardamomin from A. katsumadai has many advantages, such as facility, high efficiency, and high recovery as well.
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