[关键词]
[摘要]
目的 研究八角莲Dysosma versipellis根茎的化学成分。方法 运用硅胶、凝胶及RP-HPLC等多种色谱技术对八角莲根茎的化学成分进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果 从八角莲根茎95%乙醇提取物的醋酸乙酯和正丁醇萃取部位分离并鉴定了14个化合物,其中木脂素类化合物10个,分别为4′,5′-二去甲基鬼臼毒素(4′,5′- didemethylpodophyllotoxin,1)、鬼臼毒素(podophyllotoxin,2)、4′-去甲基鬼臼毒素(4′-demethylpodophyllotoxin,3)、山荷叶素(diphyllin,4)、鬼臼毒酮(podophyllotoxone,5)、苦鬼臼毒素葡萄糖苷(picropodophyllotoxin-4-O-β-D-glucoside,6)、4′-去甲基鬼臼毒素葡萄糖苷(4′-demethylpodophyllotoxin-4-O-β-D-glucoside,7)、山荷叶素葡萄糖苷(diphyllin-4-O-β-D- glucoside,8)、鬼臼毒素葡萄糖苷(podophyllotoxin-4-O-β-D-glucoside,9)、八角莲醇(dysosmarol,10);黄酮类化合物3个,分别为山柰酚(kaempferol,11)、槲皮素(quercetin,12)、山柰黄素-3-O-β-D-吡喃葡萄糖苷(kaempferol-3-O-β-D-glucoside,13);甾醇类化合物1个,为β-谷甾醇(β-sitosterol,14)。结论 化合物1为新天然产物,化合物7和8为首次从该属植物中分离得到。
[Key word]
[Abstract]
Objective To investigate the effect of 8-alkylberberine on glycometabolism in vitro. Methods HepG2 cells similar to human hepatic cells were used to test the glucose consumption (GC) in cultural solution in 24 h and MTT assay was used to monitor the proliferation of HepG2 cells. Results The results indicated that 8-alkyl-coptisine could increase the amounts of GC of HepG2 cells. In glucose concentration (10 mmol/L), 8-hexyl coptisine was the most significant. 8-Alkylberberine had notable inhibition in proliferation of HepG2 cells. Conclusion 8-Alkyl-coptisine was successfully synthesized. GC could increase as the length of the aliphatic chain increases firstly and the GC could decrease when the length of the aliphatic chain exceeds six atoms. 8-Hexyl coptisine is a potential hypoglycemic leading compound.
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[基金项目]
重庆市科委重大专项“黄连综合开发利用”资助项目(CSTC2008AA5021);重庆市科委攻关项目“平抑舒降糖胶囊对不同症侯糖尿病疗效及安全性研究”资助(CSTC 2010AC5007);科技部新药创制项目“黄连治疗糖尿病型高血脂并发症中药新药研究”资助(2010ZX09401-306-3-10)