[关键词]
[摘要]
目的探究一种北五味子快速繁殖的方法。方法通过正交试验对北五味子的组织培养进行研究,得出北五味子的快速繁殖的最适培养基。结果在MS培养基上附加NAA 0.3 mg/L+ZT 0.1 mg/L+6-BA 1.5 mg/L时芽的增殖效果最好而附加NAA 0.2 mg/L时,生根效果最佳。最适培养条件为温度25℃、光照强度为2 000 lx,光周期12 h。结论本研究所得最适培养基以及培养条件可用来提高北五味子产量及质量。
[Key word]
[Abstract]
Objective To develop a rapid propagation of Sehisandra chinensis.Methods The tissue culture of S.chinensis was studied by orthogonal test and the media for the rapid propagation of S.chinensis were optimized.Results When NAA 0.3,ZT 0.1,and 6-BA 1.5 mg/L were added in the MS,the best rate of bud proliferation was obtained,while the NAA 0.2 mg/L was added,the best rooting was got.The optimal cultural contion is that temperature is 25 ℃,light intensity 2 000 lx,and photoperiod 12 h.Conclusion The optimal media and cultural condition in the test could be used to improve the yield and output,as well as quality of S.chinensis.
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[基金项目]
辽宁省自然基金博士启动基金资助项目(20081067);辽宁省自然科学基金资助项目(20072124)