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[摘要]
目的探讨阿魏酸钠(sodium ferulate,SF)与苦参素(kushenin)即氧化苦参碱(oxymatrine,OMT)联合用药的协同抗炎作用。方法采用小鼠耳肿胀、大鼠足肿胀、大鼠棉球肉芽肿模型观察SF与OMT联合用药的抗炎作用。体外采用RAW264.7细胞观察联合用药对脂多糖(LPS)诱导的NO合成的影响,用Griess法测定NO生成量。结果 SF与OMT联合应用时,可明显降低二甲苯所致的小鼠耳肿胀和角叉菜胶所致的大鼠足肿胀,大剂量可减轻大鼠棉球肉芽肿的质量,但单独给予SF或OMT对上述3种炎症模型均无显著作用。体外实验结果显示,SF与OMT联合应用可明显降低LPS诱导的RAW264.7细胞NO的产生,且二者之间存在协同作用。结论 SF与OMT联合应用具有明显的协同抗炎作用,降低LPS诱导的炎性因子NO的产生,可能是其减轻炎症反应的机制之一。
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[Abstract]
Objective To assess the synergistic anti-inflammatory effect of sodium ferulate(SF) and kushenin,i.e.oxymatrine(OMT).Methods The anti-inflammatory effect of the combination of SF and OMT was evaluated on xylene-induced murine ear edema model,carrageenin-induced rat paw edema model,and rat tampon-induced granuloma model in vivo.In vitro,the effect of SF and OMT on the nitric oxide(NO) production induced by LPS was observed on RAW264.7 cells.The content of NO in the supernatant was detected with Griess reagent.Results When SF was used in combination with OMT,the edema induced by xylene and carrageenin was markedly inhibited,the weight of granuloma caused by tampon was also reduced in the high dose group,and the inhibition rate was more considerable by combined treatment than those by SF or OMT alone.In vitro,the NO production induced by LPS was depressed by combined treatment,and a synergistic anti-inflammatory effect was observed.Conclusion The present study demonstrats that the combination of SF and OMT could exert conspicuous synergistic anti-inflammatory effect,and the mechanism may be related to the inhibited effect of NO production in inflammatory factors induced by LPS.
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