目的考察止痹痛巴布剂体内外透皮给药的相关性。方法体外试验采用单室扩散池,HPLC法检测接收池中青藤碱的质量浓度体内试验检测大鼠的血药浓度,采用W-N法计算累积吸收质量浓度。对体外累积透过质量浓度与体内累积吸收质量浓度进行线性回归。结果回归方程为Y=0.097 24 X+0.323 91,r=0.955 1,体内外相关性非常显著(α=0.001)。结论此方法可用于评价透皮给药系统体内外的相关性。

Objective To investigate the correlation of transdermal delivery in vitro and in vivo with Zhibitong Cataplasm.Methods Single-chamber diffusion cell was used and sinomenine concentration was determined by HPLC in vitro.In vivo tests,W-N method was applied to calculating the cumulative absorption of concentration by plasma drug concentration in rats.And the linear regression equation was figured through the concentration of cumulative in vitro and the concentration of cumulative absorption in vivo.Results The regression equation was Y=0.097 24 X+0.323 91,r=0.955 1 and there was a high correlation between in vitro transdermal delivery and in vivo percutaneous absorption.Conclusion This method can be used to evaluate the correlation between in vitro and in vivo transdermal delivery.