目的 优化养阴清肺糖浆的生产工艺，更好地保留有效成分，提高药液的澄清度。方法 采用天然高分子絮凝剂ZTC 1＋1对养阴清肺糖浆原药水提液进行絮凝处理，以有效成分甘草酸保留率和药液浊度为衡量指标，考察絮凝剂用量、絮凝温度、搅拌速度、搅拌时间对絮凝效果的影响，并将絮凝后的药液离心处理，以确定最佳工艺条件。结果 最佳絮凝提纯工艺条件为药液温度30 ℃，ZTC 1＋1用量0.6 g/L，快搅速度450 r/min，快搅时间2 min，慢搅速度50 r/min，慢搅时间10 min，絮凝后的药液再在转速3 000 r/min下离心20 min，此时甘草酸保留率为84%，药液浊度只有1.3 NTU。结论 ZTC 1＋1用于养阴清肺糖浆原药水提液的絮凝提纯效果明显优于醇沉法，絮凝法可以替代醇沉法用于养阴清肺糖浆水提液的提纯。

Objective To optimize the production procedure of Yangyin Qingfei Syrup (YQS), improve the clarity, and reserve more active components in water extract solution of YQS. Methods The natural macromolecule flocculant ZTC 1 + 1 was applied to purify the solution of YQS. By analyzing the turbidity degree of the upper solution and the retention rate of glycyrrhizic acid, the influences of the dosage of flocculant, temperature, mixing speed, and time on flocculation effect were investigated and the optimum process conditions were obtained. Results The optimum process conditions were as follows: the flocculation temperature was 30 ℃, the dosage of ZTC 1 + 1 was 0.6 g/L, the fast mixing speed was 450 r/min, and the fast mixing time was 2 min, the slow mixing speed was 50 r/min and the slow mixing time was 10 min, then the treated solution was centrifuged for 20 min at 3 000 r/min. Under above conditions, the retention rate of glycyrrhizic acid was 84% and the turbidity degree was 1.3 NTU. Conclusion Compared with ethanol precipitation, the flocculation effect of the solution of YQS with ZTC 1 + 1 has distinct advantages. Flocculation technology can replace traditional ethanol precipitation technique for purification of the solution of YQS.

“十一五”国家重大新药创制科技重大专项综合性新药研究开发项目子课题（2009ZX09301-008）