目的 研究苦丁茶Ilex kudingcha叶的化学成分. 方法 利用硅胶柱色谱、大孔树脂柱色谱、Sephadex LH-20凝胶色谱、制备HPLC进行分离,通过波谱数据和理化性质进行化合物结构鉴定. 结果 共分离得到8个化合物,分别鉴定为mateglycoside D(1)、咖啡酸甲酯(2)、香草醛(3)、没食子酸(4)、咖啡酸(5)、β-谷甾醇(6)、对羟基苯甲醛(7)、阿魏酸(8). 结论 化合物2、7为首次从冬青属植物中分离得到,化合物1、3、4、8为首次从该植物中分离得到.

Objective To investigate the chemical constituents in the leaves of Ilex kudingcha. Methods Repeated column chromatography with silical gel, maroporous resin column, Sephadex LH-20, and preparative HPLC were used to isolate the chemical constituents, and their structures were elucidated on the basis of spectral data. Results Eight compounds were isolated and identified as mateglycoside D (1). methyl caffeate (2). vanillin (3). gallic acid (4). caffeic acid (5), β-sitosterol (6). p-hydroxy-benzaldehyde (7). and ferulic acid (8). Conclusion Compounds 2 and 7 are first obtained from the plants of Ilex L., and compounds 1, 3, 4, and 8 are first obtained from I. kudingcha.