目的 研究补阳还五汤有效部位（总苷和黄酮）对大鼠局灶性脑缺血再灌注损伤的保护作用及机制。方法 采用改良的线栓法，建立大鼠大脑中动脉栓塞（MCAO）脑缺血再灌注模型。将雄性SD大鼠随机分为假手术组、模型组、银杏叶提取物（100 mg/kg）组和补阳还五汤有效部位高、中、低剂量（200、100、50 mg/kg）组。缺血再灌注24 h后进行神经功能缺失症状评分；TTC染色法检测脑梗死体积；腹主动脉取血并离心，检测血清中乳酸脱氢酶（LDH）活性和丙二醛（MDA）水平，ELISA法检测血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）水平。结果 与模型组相比，补阳还五汤有效部位能明显改善脑缺血再灌注大鼠的神经功能障碍，减少大鼠脑组织梗死体积，显著抑制LDH活性，降低MDA和TNF-α、IL-1β、IL-6水平（P＜0.01、0.05）。结论 补阳还五汤有效部位对大鼠脑缺血再灌注损伤具有明显保护作用，其机制可能与抑制炎症因子的分泌和表达及减轻脑组织的炎症反应有关。

Objective To study the protection and mechanisms of effective fraction from Buyang Huanwu Decoction (EFBHD) on rat brain after cerebral ischemia-reperfusion injury. Methods Rat model of cerebral ischemia-reperfusion injury was created by the middle cerebral artery occlusion (MCAO) by modified suture method. Healthy male SD rats were randomly divided into six groups: Sham-operated group, model group, high-, middle-, and low-dose (200, 100, and 50 mg/kg) of EFBHD groups, and positive control group (treated with EGB 100 mg/kg). The neurological deficit symptom scores were observed and the infarct volume was measured by triphenyltetrazolium chloride (TTC) staining in 24 h after the cerebral ischemia. The contents of TNF-α, IL-1β, and IL-6 in serum were measured by enzyme-linked immunosorbent assay (ELISA) methods. In addition, the activity of LDH and MDA content in serum were determined by abdominal arterial blood and centrifuged. Results Compared with the model group, EFBHD could significantly improve the neruological dysfuction, decrease the cerebral infarct volume, and inhibit the activity of LDH, and reduce the contents of MDA, TNF-α, IL-1β, and IL-6 in serum (P<0.01, 0.05). Conclusion EFBHD has significant protection on cerebral ischemia-reperfusion injury in rats, which may be related to the inhibition of inflammatory cytokine secretion and expression and inducing the inflammation in brain tissue..

安徽省2008年度科技攻关计划重大科技专项基金（08010302099）；安徽医科大学博士基金（XJ200813）