[关键词]
[摘要]
目的 从花刺参Stichopus variegatus中分离活性皂苷类成分. 方法 将花刺参体内得到的总皂苷溶于吡啶-2-二氧六环(1:1)的混合液中,130℃加热2.5 h,应用多种色谱技术对回流产物进行分离纯化,得到2个三萜皂苷类化合物. 结果 应用现代光谱技术(2D-NMR和ESI-MS)和化学方法鉴定其结构,分别为3-O-[β-D-吡喃木糖(1→2)-4′-O-磺酸钠-β-D-吡喃木糖]-海参烷-9-烯-3β,12α,17α,25β-四醇,命名为花刺参苷A(variegatuside A,1);3-O-β-D-吡喃木糖-16β-乙酰氧基-海参烷-9-烯-22,25-环氧-3β,12α,17α-三醇,命名为花刺参苷B(variegatuside B,2). 结论 化合物1和2均为新化合物.
[Key word]
[Abstract]
To study the constituents from the sea cucumber, Stichopus variegatus. Methods The crude saponins of S. Variegatus were desulfated with pyridine-dioxane (1:1), the desulfated products were separated by multi-chromatography to afford two compounds. Results On the basis of chemical methods and spectral evidences, especially 2D-NMR and ESI-MS, these two compounds were identified as 3-O-[β-D-xylopyranosyl-(1→2)-4′-O-sulfate-β-D-xylopyranosyl]-holosta-9-ene-3β, 12α, 17α, 25β-tetraol sodium salt, named as variegatuside A (1) and 16β-acetoxy-3-O-β-D-xylopyranosyl-holosta-22, 25-epoxy-9-ene-3β, 12α, 17α-triol, named as variegatuside B (2). Conclusion Compounds 1 and 2 are new compounds.
[中图分类号]
[基金项目]
宁波市自然科学基金资助项目(2007A610083);浙江省医药卫生科学研究基金(2008B163);浙江省高校科研基金(Y200805910)