目的建立山茱萸饮片高效毛细管电泳指纹图谱分析方法。方法应用未涂渍标准熔融石英毛细管(64.5 cm×75μm,有效长度56 cm),以50 mmol/L硼砂-40 mmol/L十二烷基硫酸钠-5%乙腈(pH 9.5)为缓冲液,检测波长240 nm,运行电压10 kV,柱温18℃;以马钱苷为参照物(IS),测定指纹图谱,并做聚类分析和相似度评价。结果 初步建立了以9个共有峰为特征指纹信息的山茱萸饮片高效毛细管电泳指纹图谱,且方法学考察符合规定的标准。结论 该法准确简便,可作为控制山茱萸饮片内在质量的有效手段。

Objective To establish a micellar electrokinetic chromatography method with high performance capillary electrophoresis(HPCE) fingerprint of Corni Fructus pieces.Methods Separation was performed on a fused-silica capillary column(64.5 cm×75 μm,effective length 56 cm) with 50 mmol/L borax solution-40 mmol/L SDS-5% acetonitrile(pH 9.5) as HPCE buffer.The running voltage was 10 kV,the detection wavelength was 240 nm,and column temperature was 18 ℃.Taking loganin as reference,the data were analysed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software to compare the similarity of samples.Results HPCE Fingerprint was established with nine common peaks.Conclusion The method is accurate,simple,and suitable to the quality control of Corni Fructus.

国家自然科学基金资助项目(30873438);江苏省中药炮制重点实验室开放课题(ZYPZ007)