[关键词]
[摘要]
目的对槐定碱脂质体的处方进行考察并优化。方法硫酸铵梯度法制备槐定碱脂质体,采用单因素对影响包封率的处方因素进行初步筛选,以包封率、载药量及综合评价为指标,采用星点设计考察药物及硫酸铵浓度对制备工艺的影响,对结果进行多元线性和二项式拟合,效应面法选取最佳工艺条件进行预测分析。结果 从复相关系数上看,各指标二项式拟合方程均优于多元线性回归方程,根据优化工艺制得的槐定碱脂质体包封率为51.81%、载药量为5.39%。结论 优选的槐定碱脂质体制备工艺稳定可行。
[Key word]
[Abstract]
Objective To investigate and optimize the formulation of sophoridine liposomes.Methods Sophoridine liposomes were prepared by ammonium sulfate transmembrane gradient method.On the basis of single factor experiments,the effects of influence factors,such as entrapment efficiency,drug loading,and comprehensive indexes,were investigated by using central composite design and response surface method.The influence factors included the concentration of drug and ammonium sulfate.The data were imitated using multi-linear equation and second-order polynomial equation.Results The latter was prior to the former considering from multiple correlation coefficients.Under the optimal conditions,the entrapment efficiency and drug loading of sophoridine liposomes were 51.81% and 5.39%.Conclusion The optimized preparation technique for sophoridine liposomes is stable and feasible.
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[基金项目]
“十一五”重大新药创制项目(2009ZX09310-005);江西省自然科学基金资助项目(2008GQY0017);江西中医学院创新人才课题(JZ0909)