[关键词]
[摘要]
目的对主产区远志种质资源进行遗传多样性研究。方法利用ISSR分子标记分析来自于主产区陕西、山西和河北3个省的10个居群的远志种质资源的遗传多样性。结果 12条ISSR引物扩增出276条带,其中有271条为多态性条带,聚类分析结果表明,地理来源相同的野生居群和栽培居群先行聚合。野生和栽培远志居群的遗传变异主要发生在居群间,栽培居群的遗传多样性略低于野生居群,二者均不符合距离隔离模型。结论 聚类分析结果与栽培居群多为就地取材的事实相符。造成远志这种遗传变异型式的原因在于其本身的繁殖生物学特性以及当前的粗放栽培模式。
[Key word]
[Abstract]
Objective To discuss the genetic diversity of Polygala tenuifolia in its main production area.Methods The genetic diversity of ten populations of P.tenuifolia from its main production area Shaanxi,Shanxi,and Hebei Provinces,was analyzed using ISSR markers.Results Twelve selected ISSR primers generated a total of 276 fragments,271 of which were polymorphic fragments.The cluster analysis showed that wild and cultivated populations from the same geographical area were clustered first.Genetic variation of both wild and cultivated populations occurred mainly among populations,and the genetic diversity of cultivated populations was lower than wild ones,but both of them were not consistent with the isolation-by-distance model.Conclusion The results of cluster analysis agree with the fact that cultivated populations are derived directly from wild ones nearby.It was its own reproductive biological features and the current extensive cultivation that resulted in the genetic pattern of P.tenuifolia.Such analyses can offer necessary molecular biological reasons for the utilization and seed breeding of P.tenuifolia.
[中图分类号]
[基金项目]
长江学者和创新团队发展计划(PCSIRT);陕西省重大科技专项(2006ZK10-G3);陕西省教育厅产业化培育项目(07JC17)