目的超滤精制热毒宁处方水提液的最佳预处理方法探索及工艺研究。方法考察离心、初滤、活性炭吸附、壳聚糖絮凝等不同预处理方法对处方水提液的除杂情况以及对截留相对分子质量为3×10⁴~5×10⁴的聚砜膜超滤精制效果的影响。结果 离心、减压抽滤对降低膜污染、提高栀子苷保留率的效果不佳。活性炭吸附严重降低了栀子苷的保留率。采用壳聚糖絮凝与微滤法处理后药液的栀子苷保留率高,固含物低;采用壳聚糖絮凝比微滤法处理后续膜过程中通量大,污染度小。结论 壳聚糖絮凝法是热毒宁处方水提液的最佳预处理方法,且最佳工艺为药液温度70℃,pH 6,1%壳聚糖用量70 mL/L。

Objective To study the best pre-treatment method and its technology before ultrafiltration of Reduning water extract.Methods To investigate such pre-treatment methods as chitosan flocculation on the impurity of prescription water extract,as well as polysulfone ultrafiltration membrane refination effect with molecular weight cut-off three to five million.Results The centrifugation and decompress filtration were not benefit to promote the geniposide reservation and reduce fouling degree of membrane.Absorption of active carbon could make the geniposide reservation deeply lower.The geniposide was reserved mostly and the total extracts reduced mostly for chitosan flocculation and microfiltration.But the flux was higher and the membrane fouling is smaller after chitosan flocculation.Conclusion Chitosan flocculation is the best pre-treatment method.The operating conditions are that temperature of Reduning water extract is 70 ℃,pH value 6,and the dosage of 1% chitosan is 70 mL/L.

国家自然科学基金资助项目(3080155230873449);江苏省中管局课题(LZ09006)