[关键词]
[摘要]
目的研究绒白乳菇Lactarius vellereus的化学成分。方法用Sephadex LH-20、LH-60,硅胶以及HPLC等色谱方法分离绒白乳菇的乙醇提取物,并根据化合物的光谱数据(UV、IR、MS、1H-NMR、13C-NMR)和X晶体衍射确定其结构。结果从绒白乳菇子实体中共分离得到11个化合物,分别为isolactarorufin(1)、3-O-ethyl-lactarolide A(2)、7α,8α,13-trihydroxy-marasm-5-oic acidγ-lactone(3)、velleratretraol(4)、leptosphaepin(5)、7α,8β,13-trihydroxy-marasm-5-oic acidγ-lactone(6)、lactarolide A(7)、D-阿洛醇(8)、bis(2-ethylhexyl)phthalate(9)、硬脂酸(10)、硬脂酸甲酯(11)。经光谱分析鉴定化合物1~4和6、7为倍半萜类化合物,化合物5为生物碱类化合物,化合物8~11为常见化合物。结论化合物1、2、4、5为首次从该菌中发现的化合物。化合物4拥有高度氧化的新型倍半萜骨架;化合物5为首次从陆生生物中分离得到的一种生物碱类化合物,对研究海洋生物和陆生生物之间的进化关系具有重要的意义。
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[Abstract]
Objective To study the chemical constituents in Lactarius vellereus.Methods The compounds were extracted with ethanol and isolated by repeated column chromatography on Sephadex LH-20,LH-60,silica gel,and HPLC.Their structures were elucidated by spectroscopic methods(UV,IR,MS,1H-NMR,and 13C-NMR) and single-crystal X-Ray.Results Eleven compounds isolated from the extract of L.vellereus were isolactarorufin(1),3-O-ethyllactarolide A(2),7α,8α,13-trihydroxy-marasm-5-oic acid γ-lactone(3),velleratretraol(4),leptosphaepin(5),7α,8β,13-trihydroxy-marasm-5-oic acid γ-lactone(6),lactarolide A(7),D-allitol(8),bis(2-ethylhexyl) phthalate(9),stearic acid(10),and methyl stearate(11).Compounds 1-4,6,and 7 were identified as sesquiterpenes,and compound 5 was an alkaloid,and compounds 8-11 were common compounds.Conclusion Compounds 1,2,4,and 5 are isolated from this species for the first time.Especially,compound 4 has specially high oxidation skeleton and compound 5 is an alkaloid which is firstly separated from terrestrial biota.This is very important to study the evolution relationship between the marine life and terrestrial biota.
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[基金项目]
国家自然科学基金资助项目(30671385)