[关键词]
[摘要]
优化酶解法提取人参皂苷Rg1的工艺条件。方法采用高效液相色谱法为测定方法,以人参皂苷Rg1提取率为指标,通过正交设计试验进行优选。结果纤维素酶酶解为最优方法,且酶用量、酶解时间、酶解温度对人参皂苷Rg1提取率有显著性影响;提取人参皂苷Rg1的最佳工艺条件为:纤维素酶用量为1.4%,酶解60min,酶解温度为45℃。结论优化后的提取工艺简单、稳定、提取率高。
[Key word]
[Abstract]
optimize the enzymoly sistech nology of ginsenoside Rg1 Methods Taking ginseno side Rg1 content as the index, orthogonal design method was used for opt imization and HPLC for determ ination Results Cellulase enzy molysis was the bestex Tracting process.
[中图分类号]
[基金项目]
吉林省中医药管理局中医药科技资助项目(08SYS-102)