目的研究林荫银莲花Anemone flaccida组织培养技术,为保护与合理利用这一珍稀中药资源提供新的方法和技术途径。方法筛选适合组织培养的最佳外植体,并研究不同植物生长调节物质对林荫银莲花愈伤组织诱导和芽分化的影响。结果在MS培养基中添加2.0 mg/L 6-BA和1.0 mg/L NAA,在温度17℃条件下培养有利于愈伤组织诱导和芽的分化生长健壮的芽为最佳外植体,其愈伤组织诱导率可达85.7%,且易分化形成不定芽和拟球茎在1/2 MS+0.2 mg/L NAA培养基上,5℃条件下培养,再生拟球茎和不定芽生根良好。结论本研究为林荫银莲花种苗繁殖提供了有效途径,为其人工扩大栽培奠定了良好基础。

Objective In order to rationally protect and utilize the rare medicinal plant resources,plant tissue culture on Anemone flaccida was studied.Methods The optimal explant was screened and effects of different plant growth regulators on callus induction and bud differentiation were tested.Results The combination of 2.0 mg/L 6-BA and 1.0 mg/L NAA added in the basic MS medium was propitious to callus induction and bud differentiation.The rate of callus induction attained 85.7%,and the adventitious bud or corm-like structure was easy to differentiate when healthy bud germinating from rhizome was chosen as explant.Rooting of the regenerated adventitious bud or corm-like structure(CLS) was induced well on the 1/2MS+0.2 mg/L NAA medium under 5 ℃.ConclusionAn effective approach for the seedling propagation has been provided,which could promote artificial planting of A.flaccida greatly.

广东省教育部产学研结合项目(2006D90204009)