[关键词]
[摘要]
目的研究三桠苦Melicope pteleifolia茎的化学成分。方法运用硅胶柱色谱、反相HPLC、重结晶等方法分离纯化,并通过UV、IR、MS和NMR光谱学方法进行结构鉴定。结果从三桠苦茎中分离得到了12个化合物,分别鉴定为异吴茱萸酮酚(isoevodionol,1)、3-乙酰基-β-谷甾醇(sitost-5-en-3β-ol acetate,2)、甲基异吴茱萸酮酚(methylevodinol,3)、4-豆甾烯-3-酮(stigmast-4-en-3-one,4)、三桠苦素C(leptin C,5)、三桠苦素A(leptin A,6)、3-异戊烯基伞形花内酯[3-(3-methylbut-2-enyl)umbelliferone,7]、7-去甲基软木花椒素(7-demethylsuberosin,8)、吴茱萸春(evolitrie,9)、5-羟基-6-乙酰基-7-甲氧基色原酮(5-hydroxy-6-acety-7-methoxychromnone,10)、7α-羟基甾醇(7α-hydroxysitosterol,11)和异紫花前胡内酯(nodakenetin,12)。结论化合物2、4、7、8、10～12为首次从该植物中分离得到,化合物10为一新天然产物。
[Key word]
[Abstract]
Objective To study the chemical constitutents from the stems of Melicope pteleifolia.Methods Silica gel column chromatography,reverse phase HPLC,and recrystallization methods were used to isolate the chemical constituents,and the structures of the isolated compounds were elucidated by spectral analyses.Results Twelve compounds were isolated from EtOAc extract in stems of M.pteleifolia.The structures were identified as isoevodionol(1),sitost-5-en-3β-ol acetate(2),methylevodinol(3),stigmast-4-en-3-one(4),leptin C(5),leptin A(6),3-(3-methylbut-2-enyl) umbelliferone(7),7-demethylsuberosin(8),evolitrie(9),5-hydroxy-6-acety-7-methoxychromnone(10),7α-hydroxysitosterol(11),and nodakenetin(12),respectively.Conclusion Compounds 2,4,7,8,and 10—12 are isolated from this plant for the first time,and compound 10 is a new natural product.
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[基金项目]
广东省自然科学基金团队项目(8351063201000003);中央高校基本科研业务费专项基金(21609202);教育部新世纪优秀人才项目(NCET-08-0612)
