目的 研究佛手及属间药用植物化橘红、广陈皮的亲缘关系,同时建立了HPLC指纹图谱。方法 运用HPLC法对佛手同属的3种药用植物进行测定,对其进行主成分分析及聚类分析,实现了预分类,制定了原料药材的选择方法。结果 测定了样本的HPLC的色谱峰,从获得的17个不同产地的佛手药材的HPLC指纹图谱比较,结果从检测出17个分离度良好的共有指纹峰可以看出它们之间的相似性良好,说明佛手这一植物所含的化学成分分布及比例较稳定;使用SPSS分析软件,采用欧氏距离进行聚类分析,样品的聚类结果理想,19个样本可聚为8类,即样本1、7~10和15处于一类,样本2和3处于一类,样本4、14、16和17处于一类,样本5、6和11处于一类,样本12和样本13分别单独处于一类,而同属药用植物化橘红(样本18)和广陈皮(样本19)分别单独处于一类,可较明显地与佛手样品区分。结论 将佛手及其属间3种药用植物的中药指纹图谱及主成分聚类分析相结合,丰富了中药鉴定的理论内容。

Objective To compare the affiliation of Citrus grandis var.tomentosa fruits and C.reticulata,and establish HPLC fingerprint.Methods C.medica var.sarcodactylia fruits and three medicinal plants in Citrus L.were measured by HPLC method,which can be used to carry out principal component analysis and cluster analysis of the pre-classification of medicinal raw materials,and develope an initial method of selection.Results The HPLC chromatographic peaks of C.medica var.sarcodactylia and its adulterants were determined so as to obtain HPLC fingerprint comparison on C.medica var.sarcodactylis fruits and its adulterants from 17 different habitats.The similarity between them showed a good description of chemical distribution in this plant and stable proportion.By using the SPSS analysis software and Euclidean distance cluster analysis,sample clustering results were satisfactory.Nineteen samples can be clustered into eight categories,namely 1,7-10,and 15 samples in a class,the samples 2 and 3 in a class,samples 4,14,16,and 17 in a class,samples 5,6,and 11 are in a class,the samples 12 and 13 alone in a class,adulterants C.grandis var.tomentosa(sample 18) and C.reticulata(sample 19) left alone in a class,respectively.So it can be more obvious distinction between samples of C.medica var.sarcodatylia fruits.Conclusion The Chinese materia medica(CMM) fingerprint of C.medica var.sarcodatylia fruits and three medicinal plants in Citrus L.is combined with cluster analysis of principal components to enrich the content of CMM theory of identification.

国家“十五”重大科技专项“创新药物和中药现代化”资助项目(2001BA701A38)；广东省科技厅重点项目(2004B33001010)