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[摘要]
目的 观察与比较经曲霉菌种和根霉菌种转化后的雷公藤甲醇总提取物的药理活性和毒性反应,为筛选具有保持或提高雷公藤活性、降低毒性的生物转化菌种提供实验依据。方法 采用急性和慢性炎症模型、免疫器官质量及细胞免疫试验考察转化产物的药理活性;采用ip给药途径观察急性毒性反应。结果 曲霉菌种转化后甲醇总提取物(TW1)的抗炎、免疫抑制作用略有增强,且毒性明显降低;根霉菌种转化总提取物(TW2)毒性有所降低,但抗炎、免疫抑制作用也略有下降。结论 曲霉菌种和根霉菌种转化后的甲醇总提取物在抗炎、免疫抑制作用和急性毒性方面发生了一定的变化,减毒增效作用以曲霉菌种较为明显。
[Key word]
[Abstract]
Objective In order to reduce the toxicity and still maintain the bioactivity of the medicinal plant T ripterygium wilf ordii (TW), the whole plant biotransfomed method was employed. By observing the pharmacological activities and the toxicity of MeOH extracts from the root of T. wilf ordii that were biotransformed by the fungi Asp ergillums spp. and Rhizop us spp., the best biotransformed fungi strain has been selected based on the experimental data. Methods Applying the models of acute and chronic inflam mation, the weight of immune organ and cell immunity were used to study the pharmacological activities, and ip injection to observe the acute toxicity. Results The anti-inflammation and immunosuppression have been increased slightly and the toxicity decreased significantly after being biotransformed by A spergillus spp. (TW1), while the anti-inflammation, immunosuppression, and the toxicity have been declined after being biotransformed by Rhizopus spp. (TW2). Conclusion Although both selected fungi could change the pharmacological activities and toxicity of the T. wilf ordii, Asper gillus spp. is better than Rhizopus spp.
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[基金项目]
国家自然科学基金资助项目(30660223);云南省财政厅资助项目;云南省政府改善办学条件专项资金资助项目