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[摘要]
目的 建立龙胆药材的指纹图谱研究方法并进行聚类分析。方法 色谱柱为Shimadzu C18(3.0mm×75mm,1.7μm);流动相:乙腈-0.4%磷酸(5.5:94.5),体积流量:0.8mL/min;检测波长:240nm;柱温为40℃。结果 獐牙菜苦苷、龙胆苦苷分别在0.1548~1.858mg/mL,0.322~5.152mg/mL呈良好的线性关系,平均回收率分别为102.09%,RSD=1.68%(n=6);103.80%,RSD=1.11%(n=6)。指纹图谱的精密度与重现性试验中的各色谱峰的相对保留时间与相对峰面积的RSD小于2.1%,指纹图谱相似度结果与聚类分析谱系图一致。结论 建立的指纹图谱适用于龙胆药材的标准化种植及质量控制。
[Key word]
[Abstract]
Objective To establish method for the fingerprint of Radix et Rhizoma Gentianae using UFLC with the hierarchical cluster analysis.Methods Column:Shimadzu C18(3.0 mm×75 mm,1.7 μm);mobile phase:Acetonitrile-0.4% phosphorylation(5.5:94.5);flow rate:0.8 mL/min;detection wavelength:240 nm;temperature:40 ℃.Results This method had a good linearity in the range of 0.154 8—1.858 mg/mL,and the average recovery was 102.09% with RSD of 1.68% for swertiamarin.It had a good linearity in the range of 0.322—5.152 mg/mL,and the average recovery was 103.80% with RSD of 1.11% for gentiopicroside.The RSD of precision and reproducibility were lower than 2.1%.The result of fingerprint studying was the same with the hierarchical cluster analysis.Conclusion This method can be used as standardized implantation and quality control of Radix et Rhizoma Gentianae.
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[基金项目]
国家科技支持计划(2006DAI06A05-08)