目的 研究植物外源激素赤霉素(GA₃)和吲哚乙酸(IAA)对丹参生长和丹参酮类物质积累的影响。方法 采用温室石英砂盆栽试验与室内分析相结合的方法。结果 单独施用GA₃有利于丹参地上部分生物量的增加,但对地下部分生物量表现一定抑制作用;施用低浓度和高浓度GA₃均有利于3种丹参酮(隐丹参酮、丹参酮Ⅰ、丹参酮Ⅱ_A)的累积,施用中等浓度GA₃则不利于3种丹参酮的累积。单独施用IAA对丹参地上部分和地下部分生物量的影响呈现先升后降趋势,但统计学差异不显著,且对株高、根长的影响亦不显著;总体来看,施用低质量浓度IAA(0.5mg/L)有利于丹参根中3种丹参酮的累积,但随着IAA处理浓度增加,仅隐丹参酮量有所增加,而丹参酮Ⅰ和丹参酮Ⅱ_A量反而下降。两种激素配施时,低浓度GA₃与低浓度IAA组合处理对丹参的生长表现出显著促进作用,并能显著提高丹参根中3种丹参酮的量。结论 施用适量的植物外源激素GA₃和IAA能够促进丹参的生长和丹参根中3种丹参酮类物质的积累。

Objective To study the effects of exogenous hormones,gibberellin(GA₃)and indole-3-acetic acid(IAA),on growth and tanshinones accumulation in the radix of Salvia miltiorrhiza.Methods The herb characters and contents of tanshinones(cryptotanshinone,tanshinone Ⅰ,and tanshinone Ⅱ_A)in the radix of S.miltiorrhiza with different hormone treatments were investigated and compared in the combination of trail pot and indoor analysis.Results It promoted aboveground biomass in the radix of S.miltiorrhiza increasing with single GA₃.But it inhibited underground biomass increasing.The exogenous addition of low-level and high-level GA₃ was benefit for tanshinones accumulation,whereas the middle-level GA₃ wasn't.The aboveground and underground biomass in the radix of S.miltiorrhiza increased indistinctively with the increasing of IAA concentration and then decreased.And the IAA solutions also increased plant height and root length indistinctively.in a whole,it was benefit for tanshinones accumulation when applying low-level IAA(0.5 mg/L)singly.With the increasing of IAA concentration,the content of cryptotanshinone increased,but the contents of tanshinone Ⅰ and tanshinone Ⅱ_A decreased.It promoted S.miltiorrhiza radix growth and three tanshinones contents increasing obviously with the combined application of two low-level hormones.Conclusion The applying of GA₃ and IAA is benefit for the growth and three tanshinones accumulation in the radix of S.miltiorrhiza.

国家科技支撑计划“有效恢复中药材生产产地条件与土壤微生态环境修复技术研究”项目(2006BAI09B03)