目的 观察补阳还五汤预先干预大鼠抑制其脑死亡后心、肺炎症细胞因子表达。方法 雄性Wistar大鼠30只,体质量180~200g,随机分3组。对照组:正常大鼠麻醉后取心和肺;脑死亡模型组:诱导大鼠脑死亡;补阳还五汤组:脑死亡诱导前7d,连续每天1次给大鼠ig补阳还五汤水煎剂(1.8mL/100g,12.6g/kg)。大鼠脑死亡诱导成功,机械呼吸6h,若平均动脉压大于80mmHg,则取其心和肺。RT-PCR检测心、肺组织肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)mRNA表达,Western blotting检测心、肺组织TNF-α和IL-1β蛋白及磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)蛋白表达。结果 模型组心肺TNF-α、IL-1βmRNA和蛋白表达及p-p38MAPK蛋白表达比对照组显著增加(P<0.01)。补阳还五汤组各指标比模型组显著降低(P<0.05),但仍比对照组显著增加(P<0.01)。结论 补阳还五汤预先干预大鼠能显著抑制其脑死亡后心肺炎症细胞因子表达,可能与其在不同层面阻断p38MAPK信号通路关键靶点有关。

Objective To observe the inhibition of inflammatory cytokine expressions in hearts and lungs from brain death(BD)rats pretreated with Buyang Huanwu Decoction(BYHWT).Methods Male Wistar rats,weighing from 180 to 200 g,were randomly divided into three experimental groups:control group(n=10):hearts and lungs were gotten from normal rats after anesthesia;BD group(n=10):rat brain death was gradually induced;BYHWT+BD group(n=10):7 d before BD induction,rats were ig administered with BYHWT(1.8 mL/100 g)once daily.Six hours after successful onset of BD,only the rats whose mean arterial blood pressure were more than 80 mmHg were accepted as BD donors.Hearts and lungs were then obtained.RT-PCR was used to detect the expressions of TNF-α and IL-1β mRNA.Western blotting method was applied to determining the expressions of both TNF-α and IL-1β proteins,and the phosphorylated p38 MAPK(p-p38 MAPK)proteins.Results The expressions of mRNA and protein levels of both TNF-α and IL-1β,and also the p-p38 MAPK proteins levels,were significantly enhanced in BD group compared to the control group(P0.01).While those in BYHWT+BD group were statistically decreased(P0.05)compared to BD group,but they were still significantly increased(P0.01)in comparison with the control group.Conclusion Pretreatment of the rats with BYHWT prior to the induction of rat brain death could significantly suppress the expression of inflammatory cytokines in the hearts and lungs from BD rats.This phenomenon may relate to the blockage of key target points in p38 MAPK signaling pathway.

教育部留学回国人员科研启动基金资助项目(教外司留[2006]331)；教育部科学技术研究重点项目(206108)；国家中医药管理局留学回国人员科技活动择优资助项目(国中医药研2006LHR04号)